Employment of In Vitro Technique to Propagate Stevia (Stevia rebaudiana) Plant
نویسندگان
چکیده
Abstract The current study was conducted with aim to find an efficient simple protocol for propagation of stevia plant ( Stevia rebaudiana ) In Vitro . Shoot tips, leaves and stem nodes explants were sterilized different concentrations NaOCl (0.0, 2.0, 4.0 6% v/v) then cultured on MS medium supplemented 30 g.l -1 sucrose. Results revealed that, no contamination recorded when using % 6.0 (100% 8.9%) survival at 4.0% respectively. Moreover, combination growth regulators which used in this study, have been successfully optimized. However, best number shoots. explant found be (0.4 BAP + 0.2 Kin mg.l shoot tips average 16.44 while (2.0 0.25 GA3 mg. l produced maximum height from reached 4.85 cm. For vitro roots induction, root length roots. Shootlets (93.3 %, 9.77 9.0 cm) respectively shootlets 2.0 IBA.
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ژورنال
عنوان ژورنال: IOP conference series
سال: 2023
ISSN: ['1757-899X', '1757-8981']
DOI: https://doi.org/10.1088/1755-1315/1225/1/012025